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Bioinoculant Production for Agriculture Application


  • Banana Cultures

  • Culture Rack

  • Gladiolus cultures

  • Gladiolus cultures

Bioinoculant (biofertilizer/biopesticide) is a substance which contains living microorganisms, that enhances availability of primary nutrients to the host plant when applied or saves plants from pathogen and insect attack. It is applied to seeds, plant surfaces or soil, and colonizes the rhizosphere or the interior of the plant and promotes growth. It may constitute bacteria,  fungi,  insects and viruses. Application of microbial biofertilizers and biopesticides is being advocated by the Government and environmentalist for sustainable agriculture. However, both availability and quality of bio-inocula is confined to limited areas. Besides, there is a need to popularize the products by explaining/demonstrating their advantages over chemical products to the farmers. In spite of the thrust given to the use of biofertilizers well -trained/skilled manpower to start up small biofertilizer units is not available in the country. Therefore, we propose to develop a Skill Development course in “Bioinoculant Production for Agriculture Application”. The skilled manpower from the course should also be employable in the existing production units. The proposal is in line with the NSDC mandate  of “Creating more non-agricultural jobs under Agriculture Allied services”.



216 Hours

No. of seats



Educational qualification


Graduate in Science/ Agriculture/ Botany/ Zoology/ Microbiology/ Biotechnology

Age group


20-35 years

Course fee


Rs. 10,000 (Rs. 20,000 for industry sponsored)




The skilled personal will get opportunity in biofertilizer and biopesticide industries and self employment, addressing the issues of employment to undergraduates. Under this program, hands-on training would be provided in the field of Biofertilizer production Technology which will provide the following benefits:

  • Address the skill gaps in the field of biofertilizer/biopesticide production
  • Generation of employment
  • Generation of awareness for biofertilizers and biopesticides use
  • Help in self employment and giving impetus to entrepreneurs

    The evaluation will consist of the following components:
    a. Theory
    -Continuous assessment through assignments           :   20 marks
    - Mid- term examination                                            :   30 marks
    - Final examination                                                   :   50 marks

    b. Practical                                                                            
    -Continuous assessment through assignments         :   20 marks
    - Mid- term examination                                          :   30 marks
    - Final examination                                                 :   50 marks

    Certificate will be issued to the successful candidates by the institute under CSIR Integrated Skill Initiative or ASCI.
    Course Outline
        Topics for Theory Classes

  • Introduction to microbes used as bioinoculants
  • Understand importance of biofertilizers and biopesticides
  • Concepts of Sterilization
  • IPR issues with bioinoculants
  • Types of bioinoculants (Nitrogen fixing biofertilizers, Phosphate solubilizing biofertilizers, biopesticides)
  • Differentiation of bioinoculants with the contaminants
  • Crop specificity of bioinoculants
  • Dosage and application modes of bioinoculants
  • Preservation and storage of cultures
  • Shelf life determination and its importance
  • BIS Guidelines for production
  • BIS Guidelines for quality control
  • Learn Basics of labelling the bioinoculant packets

        Topics for Practical Classes

  • Introduction to instrumentation
  • Sterilization techniques
  • Gram’s staining, preparation of media
  • Serial dilution method
  • Techniques for sub-culturing and maintenance of cultures
  • Techniques for preservation and storage of cultures
  • Preparation of media/substrates
  • Preparation of starter cultures
  • Mass production
  • Preparation of carrier based bioinoculants and curing
  • Packaging practices
  • Storage and shelf life
  • Determination of contaminants in the product
  • Determination of CFU of the product