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"WHERE PLANT BASED RESEARCH TOUCHES LIVES THROUGH INNOVATIONS"

Phytochemicals Analysis Technician

 INTRODUCTION
Skill development programme on scientific instrumentation for quality assessment of vegetable oil, essential oil, medicinal plant, food, herbal products and metabolomics using analytical tools (HPLC, GC-MS, LC-MS, HPTLC and AAS) to be undertaken for development of phytochemical analysis technicians. Hand holds practical training to be imparted on scientific instrumentation for phytochemical analysis of medicinal and aromatic plants. The major goal of the training is to develop phytochemical analysis technicians for qualitative and quantitative estimation of biologically active major metabolites of medicinal and aromatic plants using gas chromatography (GC), gas chromatography coupled with mass spectrometry (GC-MS) and liquid chromatography HPLC), atomic absorption spectroscopy etc. The training will also include metabolite profiling of medicinal plants using GC-MS and LC-MS and HPLC to analyze both polar and non-polar metabolites of primary and secondary metabolism of the medicinal and aromatic plant species. The training will provide opportunity to the trainee in various agro, pharma and herbal industries.


Duration

:

200 Hours

No. of seats

:

15

Educational qualification

:

Science/Pharmacy/Biotech Graduates

Age group

:

20-50 years

Course fee

:

Rs. 20,000 (Rs. 30,000 for sponsored)

Residential/Non-residential

:

Non-residential

SALIENT FEATURES OF THE COURSE
Theoretical and hand on training to be provided on modern analytical tools such as Gas chromatography (GC), Liquid chromatography (LC), Mass spectrometry (MS), HPLC, HPTLC, GC-MS, LC-MS, AAS  to get opportunity as skilled technical human resource (quality analyst) in agro-industries, food industries, pharma/herbal industries and testing laboratories of related fields. The training would be provided in the field of quality assessment of vegetable oil, essential oil, medicinal plant, herbal products and metabolomics using analytical tools (HPLC, GC-MS, LC-MS, HPTLC and AAS):

  • Sample preparation
  • Extraction
  • Fractionation
  • Physico-chemical analysis
  • Operation and analysis by  GLC, HPLC, GC-MS, LC-MS, HPTLC and AAS
  • Qualitative & quantitative estimation of chemical metabolites
  • Data interpretation
  • Compilation of reports
  • Awareness for NABL accreditation
  • EVALUATION OF THE PARTICIPANTS
    Sample analysis performance of individuals

  • Sample analysis:                                 40 marks
  • Data interpretation:                            20 marks
  • Preparation of report:                                    20 marks
  • Viva Voice:                                          20 marks
  • CERTIFICATION
    Certificate will be issued to the successful candidates by the competent authority of the institute under CSIR Integrated Skill Initiative.
    Course Outline
    Medicinal plants/plant extracts/herbal formulations/products

  • Extraction, isolation and purification of biomolecules from medicinal plants by TLC and column chromatography.
  • Extraction of dyes for food and textile colors.
  • Qualitative and quantitative analysis of biomarkers by HPLC.
  • Chemical fingerprinting, qualitative and quantitative estimation of biomarkers by HPTLC.
  • Metabolite profiling of medicinal plant extracts/products by GC-MS (with sample preparation/TMS derivatization of samples).
  • Qualitative and quantitative analysis of biomarkers by GC-MS.
  • Estimation of Ash value
  • Estimation of Tannins
  • Estimation of Extractive value
  • Determination of isotope composition (13C/12C) by IR-MS.
  • Carbohydrates & Gums

  • Qualitative estimation of sugars and gums (IR, Spectrophotometer)
  • Physicochemical parameters: pH, TDS, Conductivity, Optical rotation etc.
  • Solution properties of plant gums, viscosity, tensile strength.
  • Extraction, purification of carbohydrates and gums.
  • Vegetable oils

  • Estimation of oil content (Soxhlet method).
  • Determination of Color value.
  • Determination of Specific gravity.
  • Determination of Refractive index.
  • Determination of Acid value/FFA content.
  • Estimation of fatty acid composition by Gas chromatography (GC) with sample preparation.
  • Determination of oil content by TD-NMR.
  • Essential oils

  • Estimation of oil content (Hydro distillation or Solvent extraction)
  • Determination of Optical rotation
  • Determination of Refractive index
  • Determination of specific gravity
  • Determination of Acid value
  • Essential oil profiling by GLC
  • Heavy metals by AAS.
  •