Dr. Aradhana Misra

Principal Scientist

Research Interests

Dr. Aradhana Mishra holds a PhD in Biosciences in 2007 from Rani Durgavati University, Jabalpur, India. Dr. Mishra joined CSIR-National Botanical Research Institute, Lucknow, India, as a senior scientist on January, 2013 at Division of Microbial Technology. Her research work focusedon bioinspirednano-materials synthesis and plant-microbe interaction during abiotic and biotic stresses.She has been awarded for Women Scientist fellowship award, by DST, Govt. of India in 2007 and 2011. Several major research projects have been completed by her. So far, Dr. Mishra has more than 46 publications in peer-reviewed SCI journals, seven book chapters and four patents have been granted. She is also editor in reputed journals PlOS Oneand Genetics and Molecular Biology Research. In November 2019, she was honoured with prestigious Women Scientist Award 2018 by The Biotech Research Society, India.

Dr. Aradhana Misra

Principal Scientist

Research Summary

Plant microbe interactions

Dr. Aradhana Mishra constructed a novel Trichodermafusantinvolved in nutritional value enhancement for agricultural purposes. The work was expanded by exploring Trichoderma spp. to ameliorate abiotic and biotic stresses in plants. For biotic stress amelioration, microbe (Trichoderma spp. and Bacillus spp.) based bio-formulations have been developed against various phytopathogens which causes severe yield loss in commercial and medicinally valuable plants. A large part of her recent research is on bio-formulation development for targeted disease management of the Fusarium wilt of tomato by the application of endophytic bacterium.

Apart from this, her group is also involved in the evaluation of efficacy of microbes for abiotic and biotic stresses amelioration like high CO2 condition and plant pathogen infections. The research includes exploring potent microbes (Trichoderma spp.) for mitigation of adverse effects of elevated CO2 condition on the crops especially in rice at FACE (Free Air Concentration Enrichment) setup available at CSIR-NBRI.

Green nanotechnology

She started her research work in nanotechnology by promoting green synthesis of metal nanoparticles by using Trichoderma spp. Her work is known for the rapid synthesis of gold and silver nanoparticles by using extracellular metabolites of Trichodermaspp. to enhance catalytic and antimicrobial activity. Remarkable job has been done by her in the application of biogenic Silver Nanoparticles (Ag-NP) having bactericidal activity against MDR bacteria.These bio-inspired nanoparticles were found to have higher efficacy than their chemical counterparts.

Currently, Dr. Mishra has led to product development by herbal lipid based nanoemulsion that hasstrong antimicrobial activity against phytoas well as dermal pathogens. Conclusively, her research interest istargeted and sustainable use of microbes for the betterment of society by addressing the exiting core problems.

Dr. Aradhana Misra

Principal Scientist

Publications

– Giri, V.P., Pandey, S., Kumari, M., Paswan, S., Tripathi, A., Srivastava, M., Rao, Ch. V., Katiyar, R., Nautiyal, C.S., and Mishra, A., 2019. Biogenic silver nanoparticles as an efficient contrivance for wound healing acceleration than common antiseptic medicine. FEMS Microbiology Letter, 366 (16), fnz201.

– Singh, S.P., Pandey, S., Mishra, N., Giri, V.P., Mahfooz, S., Bhattacharya, A., Kumari, M., Chouhan, P., Nautiyal, C.S., andMishra, A., 2019. Supplementation of Trichoderma improves the alteration of nutrient allocation and transporter genes expression in rice under nutrient deficiencies. Plant Physiology and Biochemistry, 143, pp. 351-363.

– Bhattacharya, A., Giri, V.P., Singh, S.P., Pandey, S., Chauhan, P., Soni, S.K., Srivastava, S., Singh, P.C., and Mishra, A., 2019. Intervention of bio-protective endophyte Bacillus tequilensis enhance physiological strength of tomato during Fusarium wilt infection. Biological Control, 139, p.104074.

– Kumari, M., Giri, V.P., Pandey, S., Kumar, M., Katiyar, R., Nautiyal, C.S., and Mishra, A., 2019. An insight into the mechanism of antifungal activity of biogenic nanoparticles than their chemical counterparts. Pesticide Biochemistry and Physiology, 157, pp.45-52.

– Mishra, A., Singh, S.P., Mahfooz, S., Shukla, R., Mishra, N., Pandey, S., Dwivedi, S., Pandey, V., Shirke, P.A., and Nautiyal, C.S., 2019. External Supplement of Impulsive Micromanager Trichoderma Helps in Combating CO₂ Stress in Rice Grown Under FACE. Plant Molecular Biology Reporter, pp.1-13.

– Mishra, A., Shukla, R., Kumari, M., Tandon, A., Singh, P.C., Dwivedi, S., Bajpai, R., and Pandey, V., (2018). Soil Amendments mitigate GHGs and affect the functional community structure of soil microflora. IJTA, ISSN 0254-8755.

– Mishra, A., Singh, S.P., Mahfooz, S., Bhattacharya, A., Mishra, N., Shirke, P.A., and Nautiyal, C.S., 2018. Bacterial endophytes modulates the withanolide biosynthetic pathway and physiological performance in Withaniasomnifera under biotic stress. Microbiological research, 212, pp.17-28.

– Mishra, A., Singh, S.P., Mahfooz, S., Singh, S.P., Bhattacharya, A., Mishra, N., and Nautiyal, C.S., 2018. Endophyte-mediated modulation of defense-related genes and systemic resistance in Withaniasomnifera (L.) Dunal under Alternariaalternata stress. Appl. Environ. Microbiology. 84(8), pp.e02845-17.

– Kumari, M., Pandey, S., Bhattacharya, A., Mishra, A., and Nautiyal, C.S., 2017. Protective role of biosynthesized silver nanoparticles against early blight disease in Solanum lycopersicum. Plant Physiology and Biochemistry, 121, pp.216-225.

– Kumari, M., Pandey, S., Mishra, S.K., Nautiyal, C.S., and Mishra, A., 2017. Effect of biosynthesized silver nanoparticles on native soil microflora via plant transport during plant–pathogen–nanoparticles interaction. 3 Biotech, 7(5), p.345.

– Mahfooz, S., Singh, S.P., Mishra, N., and Mishra, A., 2017. A comparison of microsatellites in phytopathogenicAspergillus species in order to develop markers for the assessment of genetic diversity among its isolates. Frontiers in microbiology, 8, p.1774.

– Kumari, M., Pandey, S., Mishra, A., and Nautiyal, C.S., 2017. Finding a facile way for the bacterial DNA transformation by biosynthesized gold nanoparticles. FEMS microbiology letters, 364(12).

– Mishra, A., and Nautiyal, C.S., 2018. A novel Trichodermafusant for enhancing nutritional value and defence activity in chickpea. Physiology and Molecular Biology of Plants, pp.1-12.

– Kumari, M., Shukla, S., Pandey S., Giri, V. P., Tripathi, T., Bhatia, A., Kakkar, P., Nautiyal, C. S., Mishra, A., (2017). Enhanced cellular internalization: A bactericidal mechanism more relative to biogenic nanoparticles than chemical counterparts. ACS-Applied Materials & Interfaces, 9, 4519−4533.

– Kumari, M., Pandey, S., Giri, V.P., Bhattacharya, A., Shukla, R., Mishra, A., and Nautiyal, C.S., 2017. Tailoring shape and size of biogenic silver nanoparticles to enhance antimicrobial efficacy against MDR bacteria. Microbial pathogenesis, 105, pp.346-355.

– Tripathi, P., Singh, P. C., Mishra, A., Srivastava, S., Chauhan, R., Awasthi, S., and Tripathi, R. D., (2017). Arsenic tolerant Trichoderma sp. reduces arsenic induced stress in chickpea (Cicer arietinum). Environmental Pollution, 223, 137-145.

– Kumari, M., Mishra, A., Pandey, S., Singh, S.P., Chaudhry, V., Mudiam, M.K.R., Shukla, S., Kakkar, P., and Nautiyal, C.S., 2016. Physico-chemical condition optimization during biosynthesis lead to development of improved and catalytically efficient gold nano particles. Scientific reports, 6, p.27575.

– Mahfooz, S., Singh, S.P., Rakh, R., Bhattacharya, A., Mishra, N., Singh, P.C., Chauhan, P.S., Nautiyal, C.S. and Mishra, A., 2016. A comprehensive characterization of simple sequence repeats in the sequenced Trichoderma genomes provides valuable resources for marker development. Frontiers in microbiology, 7, p.575.

– Anwar, M.S., Kapri, A., Chaudhry, V., Mishra, A., Ansari, M.W., Souche, Y., Nautiyal, C.S., Zaidi, M.G.H. and Goel, R., 2016. Response of indigenously developed bacterial consortia in progressive degradation of polyvinyl chloride. Protoplasma, 253(4), pp.1023-1032.

– Tripathi, P., Singh, P. C., Mishra, A., Tripathi, R. D., and Nautiyal, C.S., (2015). Trichoderma inoculation augments grain amino acids and mineral nutrients by modulating arsenic speciation and accumulation in chickpea (Cicer arietinum L.). Ecotoxicology and environmental safety, 117, 72-80.

– Nair, K. N., Mishra, A., Suseela, M. R., Trivedi, P. K., Sawant, S. V., and Nautiyal, C.S., (2015). Transforming plant biology to translational research applications-National Botanical Research Institute. Current Science (00113891), 108(6).

– Chaudhry, V., Bhatia, A., Bharti, S. K., Mishra, S. K., Chauhan, P. S., Mishra, A., and Nautiyal, C. S., (2015). Metabolite profiling reveals abiotic stress tolerance in Tn5 mutant of Pseudomonas putida. PloS one, 10(1), e0113487.

– Pandey, G., Chaudhary, V., Yadav, S., Chauhan, P.S., Gupta, S.S., Mishra, A.,Rawat, A.K.S. and Rao, C.V., 2014. Wound Healing Activity of PremnalatifoliaRoxb. British Journal of Pharmaceutical Research, 4(8), p.929.

– Mishra, A., Kumari, M., Pandey, S., Chaudhry, V., Gupta, K.C. and Nautiyal, C.S., 2014. Biocatalytic and antimicrobial activities of gold nanoparticles synthesized by Trichoderma sp. Bioresource technology, 166, pp.235-242.

– Tripathi, R.D., Tripathi, P., Dwivedi, S., Kumar, A., Mishra, A., Chauhan, P.S., Norton, G.J. and Nautiyal, C.S., 2014. Roles for root iron plaque in sequestration and uptake of heavy metals and metalloids in aquatic and wetland plants. Metallomics, 6(10), pp.1789-1800.

Dr. Aradhana Misra

Principal Scientist

Patents

Patent: 3 nos.

– Patent filed entitled “A novel ready to use formulation useful against soil pathogens and a process for the preparation thereof” on March 2019 by Dr. Aradhana Mishra and Ms. Arpita Bhattacharya.

– Patent application entitled “A bioinoculant composition comprising Trichoderma protoplast fusion useful to enhance nutrition value and growth of plant” has been filed byDr. Aradhana Mishraand Dr. C.S. Nautiyal, CSIRon December 12, 2011(0220NF2011) and granted in 4 countriesNo:2658961 (Great Britain), 2658961 (Europe), 2658961 (Germany patent/France patent), 9068189 (United State of America).

– Patent application entitled “An Improved system and method for water treatment” has been filed by Dr. S.P. Gautam and Aradhana Mishra May1^st2012 (1328/DEL2012).