Dr. P.K. Singh

Senior Principal Scientist

Research Interests

Dr. P.K. Singh is Senior Principle Scientist in Insect Defense Laboratory in Molecular Biology and Biotechnology Division. He is leading a team of 21 research personals including, project scientist, project consultants, research associates, Ph.D. students, project staff, and technical assistants. His group is working on genetic engineering of cotton for broad-spectrum insect resistance. The target cotton pests are whitefly (Bemisia tabaci), aphids (Aphis gossypii), cotton bollworm (Helicoverpa armigera) and leaf armyworm (Spodoptera litura).

Dr. P.K. Singh

Senior Principal Scientist

Research Summary

1.      Discovery of new insecticidal proteins and their characterization

2.      Genetic engineering of cotton for insect resistance using insecticidal genes

3.      Pathway engineering of cotton for tolerance to lepidopteran insect pests

4.      Development of GM-cotton varieties for broad-spectrum insect resistant (in collaboration with Punjab Agriculture University, Ludhiana and ICAR-Central Institute of  Cotton Research, Nagpur)

5.      Site specific integration of transgene in cotton genome

6.      Analysis of transgenic cotton lines that show superior and inheritable agronomic traits 

7.      Toxicity mechanism of insecticidal proteins

8.      Trap-cum-Death Sink for whitefly and viral diseases

9.      Analysis of plant volatiles involves in preliminary interaction of whitefly with host plant 

10.   Mass spectrometric studies of proteins

Dr. P.K. Singh

Senior Principal Scientist

Publications

1.      Sunil K. Yadav, Archana, Rahul Singh, Pradhyumna Kumar Singh andPrema G. Vasudev (2019). Insecticidal fern protein Tma12 is possibly a lytic polysaccharide monooxygenase. Planta.doi 10.1007/s00425-019-03135-0.

2.      Anoop Kumar Shukla, Santosh Kumar Upadhyay, Manisha Mishra, Sharad Saurabh, Rahul Singh, Harpal Singh, Nidhi Thakur, Preeti Rai, Paras Pandey, Aradhana L. Hans, Subhi Srivastava, Vikram Rajapure, Sunil Kumar Yadav, Mithlesh Kumar Singh, Jitendra Kumar, K. Chandrashekhar, Praveen C Verma, Ajit Pratap Singh, K N Nair, SmratiBhadauria, Muhammad Wahajuddin, Sarika Singh, Sharad Sharma, Omkar, Ram Sanmukh Upadhyay, Sirish A. Ranade, Rakesh Tuli and Pradhyumna Kumar Singh (2016). Expression of an insecticidal fern protein in cotton protects against whitefly. Nature Biotechnology. 34(10):1046- 1051.

3.      Manisha Mishra, Sharad Saurabh, Rashmi Maurya, AnubhaMudawal, Devendra Parmar and Pradhyumna Kumar Singh (2016). Proteome analysis of Bemisia tabaci suggests specific targets for RNAi mediated control. Journal of Proteomics.132: 93-102.

4.      Harpal Singh, Krishan Mohan Rai, Santosh Kumar Upadhyay, Poonam Pant, Praveen Chandra Verma, Ajit Pratap Singh and Pradhyumna Kumar Singh (2015). Transcriptome sequencing of a thalloid bryophyte; Dumortierahirsuta (Sw) Nees: assembly, annotation, and marker discovery. Scientific Reports. 5: 15350: 1-10.

5.      Nidhi Thakur, Santosh Kumar Upadhyay, Praveen C. Verma, Krishnappa Chandrashekar, Rakesh Tuli and Pradhyumna K. Singh (2014). Enhanced whitefly resistance in transgenic tobacco plants expressing double stranded RNA of v-ATPase A gene. PLOSone. 9(3) e87235.

6.      Harpal Singh, S. Dixit, P K Singh, Praveen C Verma (2013). Differential peroxidase activities in three different crops upon insect feeding. Plant Signaling Behavior. 8(10): e25615.

7.      Neeraj Kumar Dubey, Ridhi Goel, Alok Ranjan, Asif Idris, Sunil Kumar Singh, Sumit Bag, Krishnappa Chandrashekar, Kapil Deo Pandey, Pradhyumna Kumar Singh and Samir V. Sawant (2013). Comparative transcriptome analysis of Gossypium hirsutumL. in response to sap sucking insects: aphid and whitefly. BMC Genomics. 14: 241.

8.      Manoj Kumar, Anoop Kumar Shukla, Harpal Singh, Praveen C. Verma and Pradhyumna K. Singh (2013). A genotype-independent Agrobacterium mediated transformation of germinated embryo of cotton (Gossypium hirsutumL.). International Journal of Bio-Technology and Research. 3(1): 81-90.

9.      Santosh Kumar, Ashutosh Kumar Roy, Mukti Nath Mishra, Mansi Shukla, Pradhyumna Kumar Singh, Anil Kumar Tripathi (2012). RpoH2 sigma factor controls the photooxidative stress response in a non- photosynthetic rhizobacterium, AzospirilumbrasilenseSP7. Microbiology. 158: 2891-2902.

10.   Santosh Kumar Upadhyay, Seema Singh, Krishnappa Chandrashekar, Rakesh Tuli and Pradhyumna Kumar singh(2012). Compatibility of garlic (Allium sativum L.) leaf agglutinin and Cry1Ac δ-endotoxin for gene pyramiding. Applied Microbiology and Biotechnology. 93: 2365-2375.

11.   Santosh Kumar Upadhyay, Sharad Saurabh, Rahul Singh, Preeti Rai, Neeraj Kumar Dubey, K. Chandrashekar, Kuldeep Singh Negi, Rakesh Tuli and P. K. Singh (2011). Purification and characterization of a lectin with high hemagglutination property isolated from Allium altoicum. Protein J. 30(6): 374.

12.   Santosh K. Upadhyay and Pradhyumna K. Singh (2011). Role of alkaline phosphatase in insecticidal action of Cry1Ac against Helicoverpaarmigera larvae. Biotechnology letters. 33(10): 2027.

13.   Santosh Kumar Upadhyay, K. Chandrashekar, Nidhi Thakur, Praveen Chandra Verma, J. Francis Borgio, Pradhyumna Kumar Singh and Rakesh Tuli (2011). RNA interference for the control of whiteflies (Bemisia tabaci) by oral route. J. Biosci. 36(1):153-161.

14.   Santosh Kumar Upadhyay, Manisha Mishra, Harpal Singh, Amol Ranjan, Krishnappa Chandrashekar, Praveen Chandra Verma, Pradhyumna Kumar Singh andRakesh Tuli (2010). Interaction of Allium sativum leaf agglutinin (ASAL) with midgut brush border membrane vesicle proteins and its stability in Helicoverpaarmigera. Proteomics.10: 4431-4440.

15.   Siddharth Tiwari, Devesh K. Mishra, Krishnappa Chandrashekar, Pradhyumna K. Singh and Rakesh Tuli (2010). Expression of δ-endotoxin Cry1EC from inducible promoter confers insect protection in peanut (Arachis hypogaea L.). Pest Management Science. 67(2):137-145.

16.   Santosh Kumar Upadhyay, Sharad Saurabh, Preeti Rai, Rahul Singh, K. Chandrashekar, Praveen C. Verma, P. K.Singh and Rakesh Tuli (2010). SUMO fusion facilitates expression and purification of garlic leaf lectin but modifies some of its properties. J. Biotechnol. 146(1): 1-8.

Dr. P.K. Singh

Senior Principal Scientist

Patents

Patent: 6 nos.

1.      A process for preparation of a novel insecticidal chitinase toxic against whiteflies, its encoding nucleotides and application thereof

2.      Allium fistulosum leaf agglutinin recombinant protein, its encoding polynucleotide, primer and process for preparation thereof

3.      A novel δ-endotoxin protein improved for insecticidal activity and host range and a gene for its high level expression in plants

4.      Chemically synthesized artificial promoter for high level expression of transgenes

5.      A novel insecticidal protein toxic to whiteflies and lepidopteran caterpillars, toxin encoding gene and its application there off

6.      A process for preparation of a novel insecticidal chitinase toxic against whiteflies, it’s encoding nucleotides and application there off

Dr. P.K. Singh

Senior Principal Scientist

Research Scholars

Mithlesh Kumar Singh

Jyoti Singh

Shafia Siddiqui

Somnath Rahangdale

Ranjana Chauhan

Bhupendra Kumar

Bhawna Gupta

Pooja Singh

Luxmi Gupta

 

Post doctoral fellows

Kashif Mohammad

Sushil Kumar Singh

 

Project staff

Sharad Saurabh – Project Scientist

Anoop Kumar Shukla – Project Consultant

Rajesh Kumar Srivastava – Project Consultant

Abhishek Srivastava – Project Consultant

Akanksha Khare – Project Consultant

Subhi Srivastava

 

Simmi Singh

 

Dr. P.K. Singh

Senior Principal Scientist

Address

 

Molecular Biology and Biotechnology

CSIR-National Botanical Research Institute

Rana Pratap Marg, Lucknow-226001, UP, India

Phone no.: 0522-2297942 

Email: pksingh@nbri.res.in