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CSIR-NBRI Sophisticated Instrumentation Facility

During the last decade, there has been a tremendous upsurge in the study of plants as source of medicines, nutraceuticals, dietary supplements, cosmeceuticals, biopesticides and several secondary metabolites. Recently, the institute has established a CIF (Central Instrumentation Facility) with high tech and sophisticated instruments to cater the services to the institutes/organizations/individuals. CIF is NABL accreditated and well equipped for physico-chemical and analytical analysis of various plant based products. Under this facility NBRI provides the services for the analysis of essential oils, vegetable oils, herbal drugs, and heavy metals using sophisticated analytical instruments like GCMS, IRMS, Td-NMR, GLC, HPLC, HPTLC, LCMS/MS, Flash chromatography, Atomic Absorption Spectrometer, UV spectrophotometer, Supercritical Carbon dioxide extraction and Stereomicroscope etc. In addition, CSIR-NBRI has developed state-of-art infrastructure required for molecular biology as well as development of transgenic plants and their further characterization.

Equipments available with SIF

  • LCMS/MS
  • Supercritical Fluid Extraction (SCEF)
  • HPLC
  • Flash Chromatography
  • AAS
  • GLC
  • GC-MS
  • MALDI-TOF
  • IRMS
  • IRMS
  • TD-NMR
  • Plant Growth chambers
  • Typhoon Imaging Phosphoimager
  • Confocal microscope
  • HPTLC
  • SEM
  • TEM
  • Cytoflourometry
  • 96 Capillary Sequencing System
  • ICP-MS
  • Supercomputing facility-PARAM SHAVAK

Details of some of the equipments

1. Name of Equipment : GC-MS
Model : DSQ-II (Thermo Scientific)
Application : identification of chemical constituents Range of detection : ppm levels

2. Name of Equipment : IRMS 
Model : DELTA V (Thermo Scientific) Application : isotopic ratio estimation

3. Name of Equipment : TD-NMR
Model : MINISPEC (Bruker) Application : oil cont ent estimation

4. Name of Equipment : MALDI TOF/TOFMS
Model: ABI Application: High molecular weight determination

5. Name of Equipment : Super Critical Fluid Extraction
Model : Deven supercriticals Pvt. Ltd. (Technically assisted by IIT Mumbai)
Application : Tecnique of extraction by supercritical CO2 Capacity : 3 L vessel capacity

6. Name of Equipment : Atomic Absorption Spectrophotometer
Model : Perkin Elmer A Analyst 400
Application : Determination of trace element. Range of detection : ppb to ppm levels

7. Name of Equipment : High Pressure Liquid Chromatography
Model : Shimadzu (Prominence): with PDA detector
Application : Analysis of non-volatile, thermally unstable compounds (secondary metabolites) Range of detection : ppm to ppb level

8. Name of Equipment : Liquid Chromatography Mass Spectrometry 
Model : Applied Biosystem API 2000
Application : Technique for identification, confirmation and quantification of mass of different metabolites. The instrument gives the classical mass spectra using electrospray and atmospheric pressure chemical ionization The molecular weight information obtained by using either positive or negative chemical ionization mode in mass. Range of detection : ppb to ppm level

9. Name of Equipment : Tintometer 
Model : Lovibond tintometer model F Application : Determination of color value. Range of detection : Y-0.1-99.9, R-0.1-99.9, B-0.1-99.9

10. Name of Equipment : Refractometer
Model : Atago DRA1 Application : Determination of refractive index Range of detection : 1.3-1.7

11. Name of Equipment : Digital Refractometer 
Mode : Leica AR 200 Application : Determination of refractive index Range of detection : 1.3-1.7

12. Name of Equipment : Steriomicroscope Steriomicroscope
Model : Leica MZ 125 Application : Technique for magnification Range : infinity corrected 4x, 10x, 40x, (sl) and 100x (sl)

13. Name of Equipment : Gas Liquid Chromatography (GLC) GLC
Model : Hewlett Packard 6890 : FID ECD detectors Application : Analysis of essential oils, Aromachemicals vegetable oils (Fatty acid composition) and pesticides (organochlorine). Range of detection : ppb to ppm levels

14. Name of Equipment : Flash Chromatography Flash-Chromatography
Model : Biotage-SPI Flash Application : Separation and isolation of chemical constituents of extract Capacity : 100 mg-5g of extract

15. Name of Equipment : Confocal Microscope Flash-Chromatography
Model : Zeiss LSM 510

 

NABL – Accreditation

NBRI has been acccreditated / since 2008 as per the requirements of ISO-17025/ 2005 from NABL(National Accreditation Board for Calibration and Testing of Laboratories) Department of Science and Technologies (DST), Govt.of India, New Delhi .
Certificate No. T-1381
Area of Scope- Herbal drugs
Essential oils
Vegetable oils
Soil and water

Services Offered

  • Quality Assessment in terms of physic-chemical and instrumental analysis of essential oils, vegetable oils, herbal drugs and soil
  • Standardization of herbal drugs and product profiling
  • Development of new product as per the requirement of industry.

For any details, please contact
Dr. Alok Lehri
Senior Principal Scientist
Central Instrumentation Facility (CIF) (TN Khoshooo Block)
CSIR-NBRI, Rana Pratap Marg Lucknow-226001
Phone No.: 0522-2207255, 2297924
Email: a.lehri@nbri.res.in